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slc17a7 ires2 cre d strain 037512 mice  (Jackson Laboratory)

 
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    Jackson Laboratory slc17a7 ires2 cre d strain 037512 mice
    CpG depletion does not alter transgene expression but prevents AAV-induced dendritic loss (A) Immunoblot of S1 homogenates probed with antibodies against transgene product EGFP and loading control GAPDH following injection of 1 × 10 10 vg of unmodified AAV1-pCAG-FLEX-EGFP or CpG-depleted <t>AAV1-pCAG-FLEX-EGFP</t> <t>into</t> <t>Slc17a7-IRES2-Cre-D</t> mice. Each lane contains samples from a different animal. UT, untreated. (B) Densitometry shows that EGFP protein levels are indistinguishable in ipsilateral S1 of Slc17a7-IRES2-Cre-D mice injected with either CpG-depleted or unmodified virus. EGFP protein levels are normalized to GAPDH protein levels in the same lane and represented as a fold change from the AAV1-treated condition. (C) Representative cortical neurons from untreated wild-type mice or ipsilateral (injected) hemispheres of wild-type mice injected with 1 × 10 10 vg of either unmodified or CpG-depleted AAV1-pCAG-FLEX-EGFP. Scale bars, 100 μm. (D) Total dendritic length and (E) total Sholl intersections of cortical neurons from untreated wild-type mice and wild-type mice injected with 1 × 10 10 vg of either unmodified or CpG-depleted AAV1-pCAG-FLEX-EGFP. Values are represented as a ratio of injected (ipsi) normalized to uninjected (contra) hemispheres. (F–H) Sholl intersections plotted as a function of distance from the soma for cortical neurons from the same animals shown in (E). (D and E), n = 4 untreated control mice, 10–14 neurons per animal (average = 12 cells); n = 5 mice injected with unmodified AAV1-pCAG-FLEX-EGFP, 10–14 neurons per animal (average = 12 cells); n = 5 mice injected with CpG-depleted AAV1-pCAG-FLEX-EGFP, 12–15 neurons per animal (average = 13 cells). Data in (B and D–H) are represented as mean ± SD. ∗∗∗∗ p < .0001; ∗∗∗ p < .001; ∗∗ p < .01; ns, not significant. In (G), a single ∗ indicates p < 0.05, and vertically stacked ∗ indicate p < 0.01. Statistical analysis: in (B, F–H), two-tailed unpaired t test; in (D) and (E), one-way ANOVA with Tukey’s multiple comparison test.
    Slc17a7 Ires2 Cre D Strain 037512 Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slc17a7+ires2+cre+d+strain+037512+mice/calb1+cre+d+ires2/pmc13148908-216-3-11
    Average 86 stars, based on 1 article reviews
    slc17a7 ires2 cre d strain 037512 mice - by Bioz Stars, 2026-10
    86/100 stars

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    1) Product Images from "Adeno-associated virus-induced neurotoxicity is prevented by CpG depletion"

    Article Title: Adeno-associated virus-induced neurotoxicity is prevented by CpG depletion

    Journal: Molecular Therapy Advances

    doi: 10.1016/j.omta.2026.201703

    CpG depletion does not alter transgene expression but prevents AAV-induced dendritic loss (A) Immunoblot of S1 homogenates probed with antibodies against transgene product EGFP and loading control GAPDH following injection of 1 × 10 10 vg of unmodified AAV1-pCAG-FLEX-EGFP or CpG-depleted AAV1-pCAG-FLEX-EGFP into Slc17a7-IRES2-Cre-D mice. Each lane contains samples from a different animal. UT, untreated. (B) Densitometry shows that EGFP protein levels are indistinguishable in ipsilateral S1 of Slc17a7-IRES2-Cre-D mice injected with either CpG-depleted or unmodified virus. EGFP protein levels are normalized to GAPDH protein levels in the same lane and represented as a fold change from the AAV1-treated condition. (C) Representative cortical neurons from untreated wild-type mice or ipsilateral (injected) hemispheres of wild-type mice injected with 1 × 10 10 vg of either unmodified or CpG-depleted AAV1-pCAG-FLEX-EGFP. Scale bars, 100 μm. (D) Total dendritic length and (E) total Sholl intersections of cortical neurons from untreated wild-type mice and wild-type mice injected with 1 × 10 10 vg of either unmodified or CpG-depleted AAV1-pCAG-FLEX-EGFP. Values are represented as a ratio of injected (ipsi) normalized to uninjected (contra) hemispheres. (F–H) Sholl intersections plotted as a function of distance from the soma for cortical neurons from the same animals shown in (E). (D and E), n = 4 untreated control mice, 10–14 neurons per animal (average = 12 cells); n = 5 mice injected with unmodified AAV1-pCAG-FLEX-EGFP, 10–14 neurons per animal (average = 12 cells); n = 5 mice injected with CpG-depleted AAV1-pCAG-FLEX-EGFP, 12–15 neurons per animal (average = 13 cells). Data in (B and D–H) are represented as mean ± SD. ∗∗∗∗ p < .0001; ∗∗∗ p < .001; ∗∗ p < .01; ns, not significant. In (G), a single ∗ indicates p < 0.05, and vertically stacked ∗ indicate p < 0.01. Statistical analysis: in (B, F–H), two-tailed unpaired t test; in (D) and (E), one-way ANOVA with Tukey’s multiple comparison test.
    Figure Legend Snippet: CpG depletion does not alter transgene expression but prevents AAV-induced dendritic loss (A) Immunoblot of S1 homogenates probed with antibodies against transgene product EGFP and loading control GAPDH following injection of 1 × 10 10 vg of unmodified AAV1-pCAG-FLEX-EGFP or CpG-depleted AAV1-pCAG-FLEX-EGFP into Slc17a7-IRES2-Cre-D mice. Each lane contains samples from a different animal. UT, untreated. (B) Densitometry shows that EGFP protein levels are indistinguishable in ipsilateral S1 of Slc17a7-IRES2-Cre-D mice injected with either CpG-depleted or unmodified virus. EGFP protein levels are normalized to GAPDH protein levels in the same lane and represented as a fold change from the AAV1-treated condition. (C) Representative cortical neurons from untreated wild-type mice or ipsilateral (injected) hemispheres of wild-type mice injected with 1 × 10 10 vg of either unmodified or CpG-depleted AAV1-pCAG-FLEX-EGFP. Scale bars, 100 μm. (D) Total dendritic length and (E) total Sholl intersections of cortical neurons from untreated wild-type mice and wild-type mice injected with 1 × 10 10 vg of either unmodified or CpG-depleted AAV1-pCAG-FLEX-EGFP. Values are represented as a ratio of injected (ipsi) normalized to uninjected (contra) hemispheres. (F–H) Sholl intersections plotted as a function of distance from the soma for cortical neurons from the same animals shown in (E). (D and E), n = 4 untreated control mice, 10–14 neurons per animal (average = 12 cells); n = 5 mice injected with unmodified AAV1-pCAG-FLEX-EGFP, 10–14 neurons per animal (average = 12 cells); n = 5 mice injected with CpG-depleted AAV1-pCAG-FLEX-EGFP, 12–15 neurons per animal (average = 13 cells). Data in (B and D–H) are represented as mean ± SD. ∗∗∗∗ p < .0001; ∗∗∗ p < .001; ∗∗ p < .01; ns, not significant. In (G), a single ∗ indicates p < 0.05, and vertically stacked ∗ indicate p < 0.01. Statistical analysis: in (B, F–H), two-tailed unpaired t test; in (D) and (E), one-way ANOVA with Tukey’s multiple comparison test.

    Techniques Used: Expressing, Western Blot, Control, Injection, Virus, Two Tailed Test, Comparison

    Related Articles

    Expressing:

    Article Title: Adeno-associated virus-induced neurotoxicity is prevented by CpG depletion
    Article Snippet: Male C57BL/6J and Slc17a7-IRES2-Cre-D (Strain # 037512) mice were obtained from Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME).

    Article Title: Adeno-Associated Virus-Induced Neurotoxicity is Prevented by CpG Depletion
    Article Snippet: Male C57BL/6J and Slc17a7-IRES2-Cre-D (Strain # 037512) mice were obtained from Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME).

    Western Blot:

    Article Title: Adeno-associated virus-induced neurotoxicity is prevented by CpG depletion
    Article Snippet: Male C57BL/6J and Slc17a7-IRES2-Cre-D (Strain # 037512) mice were obtained from Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME).

    Article Title: Adeno-Associated Virus-Induced Neurotoxicity is Prevented by CpG Depletion
    Article Snippet: Male C57BL/6J and Slc17a7-IRES2-Cre-D (Strain # 037512) mice were obtained from Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME).

    Control:

    Article Title: Adeno-associated virus-induced neurotoxicity is prevented by CpG depletion
    Article Snippet: Male C57BL/6J and Slc17a7-IRES2-Cre-D (Strain # 037512) mice were obtained from Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME).

    Article Title: Adeno-Associated Virus-Induced Neurotoxicity is Prevented by CpG Depletion
    Article Snippet: Male C57BL/6J and Slc17a7-IRES2-Cre-D (Strain # 037512) mice were obtained from Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME).

    Injection:

    Article Title: Adeno-associated virus-induced neurotoxicity is prevented by CpG depletion
    Article Snippet: Male C57BL/6J and Slc17a7-IRES2-Cre-D (Strain # 037512) mice were obtained from Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME).

    Article Title: Adeno-Associated Virus-Induced Neurotoxicity is Prevented by CpG Depletion
    Article Snippet: Male C57BL/6J and Slc17a7-IRES2-Cre-D (Strain # 037512) mice were obtained from Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME).

    Virus:

    Article Title: Adeno-associated virus-induced neurotoxicity is prevented by CpG depletion
    Article Snippet: Male C57BL/6J and Slc17a7-IRES2-Cre-D (Strain # 037512) mice were obtained from Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME).

    Article Title: Adeno-Associated Virus-Induced Neurotoxicity is Prevented by CpG Depletion
    Article Snippet: Male C57BL/6J and Slc17a7-IRES2-Cre-D (Strain # 037512) mice were obtained from Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME).

    Two Tailed Test:

    Article Title: Adeno-associated virus-induced neurotoxicity is prevented by CpG depletion
    Article Snippet: Male C57BL/6J and Slc17a7-IRES2-Cre-D (Strain # 037512) mice were obtained from Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME).

    Article Title: Adeno-Associated Virus-Induced Neurotoxicity is Prevented by CpG Depletion
    Article Snippet: Male C57BL/6J and Slc17a7-IRES2-Cre-D (Strain # 037512) mice were obtained from Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME).

    Comparison:

    Article Title: Adeno-associated virus-induced neurotoxicity is prevented by CpG depletion
    Article Snippet: Male C57BL/6J and Slc17a7-IRES2-Cre-D (Strain # 037512) mice were obtained from Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME).

    Article Title: Adeno-Associated Virus-Induced Neurotoxicity is Prevented by CpG Depletion
    Article Snippet: Male C57BL/6J and Slc17a7-IRES2-Cre-D (Strain # 037512) mice were obtained from Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME).



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    slc17a7 ires2 cre d strain 037512 mice  (Jackson Laboratory)
    86
    Jackson Laboratory slc17a7 ires2 cre d strain 037512 mice
    CpG depletion does not alter transgene expression but prevents AAV-induced dendritic loss (A) Immunoblot of S1 homogenates probed with antibodies against transgene product EGFP and loading control GAPDH following injection of 1 × 10 10 vg of unmodified AAV1-pCAG-FLEX-EGFP or CpG-depleted <t>AAV1-pCAG-FLEX-EGFP</t> <t>into</t> <t>Slc17a7-IRES2-Cre-D</t> mice. Each lane contains samples from a different animal. UT, untreated. (B) Densitometry shows that EGFP protein levels are indistinguishable in ipsilateral S1 of Slc17a7-IRES2-Cre-D mice injected with either CpG-depleted or unmodified virus. EGFP protein levels are normalized to GAPDH protein levels in the same lane and represented as a fold change from the AAV1-treated condition. (C) Representative cortical neurons from untreated wild-type mice or ipsilateral (injected) hemispheres of wild-type mice injected with 1 × 10 10 vg of either unmodified or CpG-depleted AAV1-pCAG-FLEX-EGFP. Scale bars, 100 μm. (D) Total dendritic length and (E) total Sholl intersections of cortical neurons from untreated wild-type mice and wild-type mice injected with 1 × 10 10 vg of either unmodified or CpG-depleted AAV1-pCAG-FLEX-EGFP. Values are represented as a ratio of injected (ipsi) normalized to uninjected (contra) hemispheres. (F–H) Sholl intersections plotted as a function of distance from the soma for cortical neurons from the same animals shown in (E). (D and E), n = 4 untreated control mice, 10–14 neurons per animal (average = 12 cells); n = 5 mice injected with unmodified AAV1-pCAG-FLEX-EGFP, 10–14 neurons per animal (average = 12 cells); n = 5 mice injected with CpG-depleted AAV1-pCAG-FLEX-EGFP, 12–15 neurons per animal (average = 13 cells). Data in (B and D–H) are represented as mean ± SD. ∗∗∗∗ p < .0001; ∗∗∗ p < .001; ∗∗ p < .01; ns, not significant. In (G), a single ∗ indicates p < 0.05, and vertically stacked ∗ indicate p < 0.01. Statistical analysis: in (B, F–H), two-tailed unpaired t test; in (D) and (E), one-way ANOVA with Tukey’s multiple comparison test.
    Slc17a7 Ires2 Cre D Strain 037512 Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slc17a7+ires2+cre+d+strain+037512+mice/calb1+cre+d+ires2/pmc13148908-216-3-11
    Average 86 stars, based on 1 article reviews
    slc17a7 ires2 cre d strain 037512 mice - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    CpG depletion does not alter transgene expression but prevents AAV-induced dendritic loss (A) Immunoblot of S1 homogenates probed with antibodies against transgene product EGFP and loading control GAPDH following injection of 1 × 10 10 vg of unmodified AAV1-pCAG-FLEX-EGFP or CpG-depleted AAV1-pCAG-FLEX-EGFP into Slc17a7-IRES2-Cre-D mice. Each lane contains samples from a different animal. UT, untreated. (B) Densitometry shows that EGFP protein levels are indistinguishable in ipsilateral S1 of Slc17a7-IRES2-Cre-D mice injected with either CpG-depleted or unmodified virus. EGFP protein levels are normalized to GAPDH protein levels in the same lane and represented as a fold change from the AAV1-treated condition. (C) Representative cortical neurons from untreated wild-type mice or ipsilateral (injected) hemispheres of wild-type mice injected with 1 × 10 10 vg of either unmodified or CpG-depleted AAV1-pCAG-FLEX-EGFP. Scale bars, 100 μm. (D) Total dendritic length and (E) total Sholl intersections of cortical neurons from untreated wild-type mice and wild-type mice injected with 1 × 10 10 vg of either unmodified or CpG-depleted AAV1-pCAG-FLEX-EGFP. Values are represented as a ratio of injected (ipsi) normalized to uninjected (contra) hemispheres. (F–H) Sholl intersections plotted as a function of distance from the soma for cortical neurons from the same animals shown in (E). (D and E), n = 4 untreated control mice, 10–14 neurons per animal (average = 12 cells); n = 5 mice injected with unmodified AAV1-pCAG-FLEX-EGFP, 10–14 neurons per animal (average = 12 cells); n = 5 mice injected with CpG-depleted AAV1-pCAG-FLEX-EGFP, 12–15 neurons per animal (average = 13 cells). Data in (B and D–H) are represented as mean ± SD. ∗∗∗∗ p < .0001; ∗∗∗ p < .001; ∗∗ p < .01; ns, not significant. In (G), a single ∗ indicates p < 0.05, and vertically stacked ∗ indicate p < 0.01. Statistical analysis: in (B, F–H), two-tailed unpaired t test; in (D) and (E), one-way ANOVA with Tukey’s multiple comparison test.

    Journal: Molecular Therapy Advances

    Article Title: Adeno-associated virus-induced neurotoxicity is prevented by CpG depletion

    doi: 10.1016/j.omta.2026.201703

    Figure Lengend Snippet: CpG depletion does not alter transgene expression but prevents AAV-induced dendritic loss (A) Immunoblot of S1 homogenates probed with antibodies against transgene product EGFP and loading control GAPDH following injection of 1 × 10 10 vg of unmodified AAV1-pCAG-FLEX-EGFP or CpG-depleted AAV1-pCAG-FLEX-EGFP into Slc17a7-IRES2-Cre-D mice. Each lane contains samples from a different animal. UT, untreated. (B) Densitometry shows that EGFP protein levels are indistinguishable in ipsilateral S1 of Slc17a7-IRES2-Cre-D mice injected with either CpG-depleted or unmodified virus. EGFP protein levels are normalized to GAPDH protein levels in the same lane and represented as a fold change from the AAV1-treated condition. (C) Representative cortical neurons from untreated wild-type mice or ipsilateral (injected) hemispheres of wild-type mice injected with 1 × 10 10 vg of either unmodified or CpG-depleted AAV1-pCAG-FLEX-EGFP. Scale bars, 100 μm. (D) Total dendritic length and (E) total Sholl intersections of cortical neurons from untreated wild-type mice and wild-type mice injected with 1 × 10 10 vg of either unmodified or CpG-depleted AAV1-pCAG-FLEX-EGFP. Values are represented as a ratio of injected (ipsi) normalized to uninjected (contra) hemispheres. (F–H) Sholl intersections plotted as a function of distance from the soma for cortical neurons from the same animals shown in (E). (D and E), n = 4 untreated control mice, 10–14 neurons per animal (average = 12 cells); n = 5 mice injected with unmodified AAV1-pCAG-FLEX-EGFP, 10–14 neurons per animal (average = 12 cells); n = 5 mice injected with CpG-depleted AAV1-pCAG-FLEX-EGFP, 12–15 neurons per animal (average = 13 cells). Data in (B and D–H) are represented as mean ± SD. ∗∗∗∗ p < .0001; ∗∗∗ p < .001; ∗∗ p < .01; ns, not significant. In (G), a single ∗ indicates p < 0.05, and vertically stacked ∗ indicate p < 0.01. Statistical analysis: in (B, F–H), two-tailed unpaired t test; in (D) and (E), one-way ANOVA with Tukey’s multiple comparison test.

    Article Snippet: Male C57BL/6J and Slc17a7-IRES2-Cre-D (Strain # 037512) mice were obtained from Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME).

    Techniques: Expressing, Western Blot, Control, Injection, Virus, Two Tailed Test, Comparison